This study examined the effects of cyclosporin A(CsA) and methylprednisolone(MP) on the viability of the devascularized trachea after heterotopic transplantation.
Fourty-two tracheal segments were harvested from 21 donor Wistar rats. Those tracheal segments were heterotopically implanted into the abdomen of recipient rats after wrapping in omentum. Heterotopical implantation was performed in six groups of
rats:
Group I was Wistar syngeneic controls, and five groups of Sprague Dawley recipients, receiving no immunosuppression(Group II), CsA alone (Group III, V), and CsA in combination with MP(Group IV,VI), After 14 days, the tracheal segments were
histologically evaluated.
Epithelial thickness and the degree of epithelial regeneration were significantly different between group I and group II, III, VI, VI(p<0.05). There were significant differences in the epithelial thickness between group II, III, IV and group V,
VI,
In
the degree of epithelial regeneration, there were significant differences in group II, group III-IV, and group V-VI. Without immunosuppression there was virtually no epithelium, whereas low-dose immunosuppression yielded intermediated viability,
and
with high dose CsA and MP we observed improved tracheal viability.
Our results suggest that optimal combination of CsA and MP may improve the viability in heterotopic tracheal allografts. (Korean J Thoracic Cardiovas Surg 1994; 27:15-23)
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